Device

Part:BBa_K2018049

Designed by: Joel Vej-Nielsen   Group: iGEM16_SDU-Denmark   (2016-10-13)


BioBrick that produces bioplastic and secretes it

The biobrick contains phaCAB with a hemolysin based type I secretion system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 981
    Illegal BglII site found at 1806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 287
    Illegal NgoMIV site found at 358
    Illegal NgoMIV site found at 958
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 1549
    Illegal NgoMIV site found at 2201
    Illegal NgoMIV site found at 2223
    Illegal AgeI site found at 6620
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4067
    Illegal SapI.rc site found at 8477

The BioBrick encodes the inner membrane transport complex proteins HlyB and HlyD. These transport proteins, derived from Escherichia coli strain SEC470. The BioBrick also encodes part of the hemolysin toxin, HlyA, fused with phasin, a protein that binds to PHB granules. Furthermore the BioBrick encodes phaCAB, so it is able to produce and secrete PHB.


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Categories
Parameters
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